Introduction: An acute bout of exercise is associated with an increased cognitive performance and oxygenation levels in the cortical areas of human brain. Aim of the study: In the current study we evaluated the effect of acute bout of highintensity intermittent exercise (HIIE) on choice reaction time (CRT) and the associated changes in the prefrontal cortex (PFC) hemodynamic function in young adults. Material and methods: Twenty-five participants (12 males and 13 females) were included in a randomized crossover trial with control and HIIE sessions in a counterbalanced order. (VTS) was used to assess CRT during various sessions. Functional near-infrared spectroscopy system was used to evaluate cortical hemodynamic changes during the cognitive task. Results: Results showed a significant decrease in CRT (p textless 0.001) and motor time (p textless 0.001) following the HIIE intervention. HIIE showed a significant (p textless 0.05) effect on hemodynamics of bilateral dorsolateral PFC and bilateral frontopolar areas of PFC. Conclusions: CRT performance is improved in response to a single session of HIIE. These improvements are associated with the changes in hemodynamics of PFC. This information helps to understand relationship between HIIE, cognitive domains and its associated PFC activation.