Purpose: Resting-state functional connectivity in subjects with cerebral infarction (CI) was assessed using wavelet-based coherence analysis of near-infrared spectroscopy (NIRS) signals. Methods: Continuous recordings of NIRS signals were measured from the prefrontal cortex and sensorimotor cortical areas of 12 subjects with CI (CI group) and 16 healthy subjects (healthy group) during the resting state. The channels in these areas were divided into four connection types: homologous connectivity, frontoposterior connectivity, contralateral connectivity, and homolateral connectivity. Wavelet coherence (WCO) and wavelet phase coherence (WPCO) were calculated in six frequency intervals in each channel pair: I, 0.62 Hz; II, 0.1450.6 Hz; III, 0.0520.145 Hz; IV, 0.0210.052 Hz; V, 0.00950.021 Hz; and VI, 0.0050.0095 Hz. Results: WCO in the six frequency intervals was significant for all channels in the healthy group. By contrast, WCO in frequency intervals IIVI showed weakened connectivity in the CI group, especially in terms of frontoposterior connectivity. WCO was significantly lower in the CI group than in the healthy group in the following connectivities and frequency intervals: front-posterior, IVVI (p < 0.05); homologous, IIIV (p < 0.01); motor-contralateral, IIIV (p < 0.05); and motorhomolateral, IIIV (p < 0.05). WPCO in frequency intervals III (F = 5.032, p = 0.033) and IV (F = 11.95, p = 0.002) in frontoposterior connectivity, as well as in intervals IIIV in homologous, motor-contralateral and motor-homolateral connectivities were significantly lower (p < 0.05) in the CI group than in the healthy group. However, WPCO in interval I showed significantly higher levels in motor-homolateral connectivity in the CI group than in the healthy group (F = 4.241, p = 0.049). Conclusions: The authors results suggest that CI causes a frequency-specific disruption in restingstate connectivity. This may be useful for assessing the effectiveness of functional recovery after CI.